1/15/2024 0 Comments Ema arai![]() ![]() ![]() (C) Expression of MHC class I evaluated by reverse-transcription polymerase chain reaction (RT-PCR). The cells convert to epistem-like cells following withdrawal of pLIF and forskolin and addition of basic fibroblast growth factor (bFGF). (B) iPSCs stop growing following withdrawal of porcine leukemia inhibitory factor (pLIF) and forskolin. The cells are positive for alkaline phosphatase (ALPase) activity (b). (A) Porco Rosso iPSCs form compact and round colonies and express the humanized Kusabira-orange (huKO) fluorescence protein (a). Putative naive porcine iPSCs are LIF-dependent and MHC class I-negative. This is the first demonstration of macroscopic fluorescent chimeras derived from naive-like porcine PSCs, although adult chimeras remain to be produced. They also contributed to the in utero fetal development (11/71, 15.5% at day 23 1/13, 7.7% at day 65). ![]() The porcine iPSCs contributed to the in vitro embryonic development (11/24, 45.8%) as assessed by fluorescent markers. The naive state of the cells was demonstrated by pLIF dependency, 2 active X chromosomes (when female), absent MHC class I expression, and characteristic gene expression profiles. The cells expressed pluripotency markers and formed embryoid bodies, which gave rise to cell types from all 3 embryonic germ layers. Emergent colonies were propagated in the presence of porcine leukemia inhibitory factor (pLIF) and forskolin. Porcine embryonic fibroblasts were transduced with retroviral vectors expressing Yamanaka's 4 genes. In this study, putative naive porcine-induced pluripotent stem cells (iPSCs) were generated. Previously reported porcine PSCs appear in the primed state. Mouse PSCs are naive, but human PSCs are in the primed state. Only the naive type has the capacity for producing chimeric offspring. In pluripotent stem cells (PSCs), there are 2 types: naive and primed. ![]()
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